Abstract
BackgroundBovine tuberculosis is a highly prevalent infectious disease of cattle worldwide; however, infection in the United States is limited to 0.01% of dairy herds. Thus detection of bovine TB is confounded by high background infection with M. avium subsp. paratuberculosis. The present study addresses variations in the circulating peptidome based on the pathogenesis of two biologically similar mycobacterial diseases of cattle.Methodology/Principal FindingsWe hypothesized that serum proteomes of animals in response to either M. bovis or M. paratuberculosis infection will display several commonalities and differences. Sera prospectively collected from animals experimentally infected with either M. bovis or M. paratuberculosis were analyzed using high-resolution proteomics approaches. iTRAQ, a liquid chromatography and tandem mass spectrometry approach, was used to simultaneously identify and quantify peptides from multiple infections and contemporaneous uninfected control groups. Four comparisons were performed: 1) M. bovis infection versus uninfected controls, 2) M. bovis versus M. paratuberculosis infection, 3) early, and 4) advanced M. paratuberculosis infection versus uninfected controls. One hundred and ten differentially elevated proteins (P≤0.05) were identified. Vitamin D binding protein precursor (DBP), alpha-1 acid glycoprotein, alpha-1B glycoprotein, fetuin, and serine proteinase inhibitor were identified in both infections. Transthyretin, retinol binding proteins, and cathelicidin were identified exclusively in M. paratuberculosis infection, while the serum levels of alpha-1-microglobulin/bikunin precursor (AMBP) protein, alpha-1 acid glycoprotein, fetuin, and alpha-1B glycoprotein were elevated exclusively in M. bovis infected animals.Conclusions/SignificanceThe discovery of these biomarkers has significant impact on the elucidation of pathogenesis of two mycobacterial diseases at the cellular and the molecular level and can be applied in the development of mycobacterium-specific diagnostic tools for the monitoring progression of disease, response to therapy, and/or vaccine based interventions.
Highlights
Bovine tuberculosis, a zoonotic infection in cattle caused by the intracellular bacterium Mycobacterium bovis (M. bovis), is a highly prevalent infectious disease of cattle worldwide [1]
M. bovis is wide spread, bovine tuberculosis is limited to 0.01% of dairy herds in the U.S This is in stark contrast to another mycobacterial disease of cattle, Johne’s Disease (JD), which afflicts 68% of U.S dairy herds (2007, USDA, report)
Bovine TB control and eradication depends on the development of tests capable of detecting early infection and discriminating bovine TB from M. paratuberculosis
Summary
Bovine tuberculosis (bovine TB), a zoonotic infection in cattle caused by the intracellular bacterium Mycobacterium bovis (M. bovis), is a highly prevalent infectious disease of cattle worldwide [1]. Human tuberculosis caused by M. bovis is underestimated and the evidence that animal-human and inter-human transmission can occur underscores the importance of undertaking control programs based on robust understanding of the pathogenesis of the disease [2,3,4]. M. bovis detection is confounded by background infection with M. paratuberculosis (MAP), the causative agent of JD. Bovine tuberculosis is a highly prevalent infectious disease of cattle worldwide; infection in the United States is limited to 0.01% of dairy herds. The present study addresses variations in the circulating peptidome based on the pathogenesis of two biologically similar mycobacterial diseases of cattle
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
