Biomanufacturing Harvest Optimization With Small Data

Abstract

In biopharmaceutical manufacturing, fermentation processes play a critical role in productivity and profit. A fermentation process uses living cells with complex biological mechanisms, leading to high variability in the process outputs, namely, the protein and impurity levels. By building on the biological mechanisms of protein and impurity growth, we introduce a stochastic model to characterize the accumulation of the protein and impurity levels in the fermentation process. However, a common challenge in the industry is the availability of only a very limited amount of data, especially in the development and early stages of production. This adds an additional layer of uncertainty, referred to as model risk, due to the difficulty of estimating the model parameters with limited data. In this article, we study the harvesting decision for a fermentation process (i.e., when to stop the fermentation and collect the production reward) under model risk. We adopt a Bayesian approach to update the unknown parameters of the growth-rate distributions, and use the resulting posterior distributions to characterize the impact of model risk on fermentation output variability. The harvesting problem is formulated as a Markov decision process model with knowledge states that summarize the posterior distributions and hence incorporate the model risk in decision-making. Our case studies at MSD Animal Health demonstrate that the proposed model and solution approach improve the harvesting decisions in real life by achieving substantially higher average output from a fermentation batch along with lower batch-to-batch variability.