Bioluminescence assays for bile acids in the detection and follow-up of experimental liver injury.

Abstract

We evaluated the usefulness of recently developed bioluminescence assays for serum bile acids (BA) in the detection and follow-up of experimental liver injury. Liver damage was induced in rats by either D-galactosamine or CCl4, and BA were compared to SGPT and aminopyrine breath test (ABT). In severe liver injury, following D-galactosamine administration, all three methods revealed a significant difference from control values. The degree of abnormality was, however, far greater with SGPT and BA than with ABT. In moderate liver injury, induced by CCl4, the increase in BA was not significant. Values of SGPT and BA showed a very good correlation (3 alpha-OH: r = 0.88; 7 alpha-OH: r = 0.90; 12 alpha-OH: r = 0.83; p less than 0.001 for all correlations). Application of different assays for 3 alpha-OH, 7 alpha-OH and 12 alpha-OH BA allowed us to assess changes in individual BA. A 96-hr follow-up study in D-galactosamine-treated animals showed an increase in BA up to 48 hr and a decrease thereafter. The bioluminescence assays for BA are simple, rapid and require only 10 microliter of serum. Thus, these assays may be the method of choice in detecting and monitoring liver injury in small laboratory animals.