Biology of simian virus 40 (SV40) transplantation antigen (TrAg) VII. Induction of SV40 TrAg in nonpermissive mouse cells by early viable SV40 deletion (0.54/0.59) mutants

Abstract

Early viable deletion (0.54/0.59) mutants were tested for their ability to induce SV40 transplantation antigen (TrAg) in infected or transformed nonpermissive mouse cells in an attempt to determine the requirement for small t antigen in the expression of TrAg at the cell surface. The results indicate that dl (0.54/0.59) mutants are as efficient as wild-type SV40 in generating specific cytotoxic lymphocytes in C57BU6 mice and in immunizing BALB/c mice against an SV40 tumor cell challenge. Mouse (C57BU6) cells transformed by these mutants were also susceptible to lysis by the specifically sensitized lymphocytes. It can, therefore, be concluded that the synthesis of small t antigen is not an absolute requirement for expression of SV40 TrAg in SV40-infected or -transformed nonpermissive cells.