Abstract
Seven Colletotrichum acutatum strains isolated from strawberries were cultivated on various culture media and tested in vitro and in vivo on Fragaria x ananassa for anthracnose symptoms. PDA caused an optimum growth of all isolates, MEA, ML and Strawberry allowed a good but not optimal growth. Czapeck, Sabouraud and the organic medium from potato gave the lowest growth rate of all isolates. PDA allowed a good sporulation of isolates follow-up by strawberry, MEA; in contrast, ML, Czapeck and Sabouraud gave a low sporulation. The fungal development is maximal at 25°C and 27°C for all the isolates studied. No growth was observed at 5°C and 37°C. The studied strains developed at all pH values. They didn’t develop at 0.6, 0.65 and 0.7 aw but mycelial growth was perfect at 1 and 0.95 aw. The in vitro test of the pathogenicity caused by C. acutatum strains on strawberry’s leaves showed an increasing percentage of infection with time and different infection rates among C. acutatum strains, strain Ca6 having a pathogenic power very high compared to the other isolates. After in vivo inoculation and incubation of the seedlings, all isolates caused severe symptoms related to anthracnose on leaflets and petioles of the studied strawberry plants.
Highlights
Strawberry plant, Fragaria x ananassa, is one of the most widespread horticultural crop in the world
The in vitro test of the pathogenicity caused by C. acutatum strains on strawberry’s leaves showed an increasing percentage of infection with time and different infection rates among C. acutatum strains, strain Ca6 having a pathogenic power very high compared to the other isolates
Colletotrichum acutatum strains cause symptoms related to the anthracnose in vitro and in vivo but the severity of the disease varies from an isolate to another, isolate Ca6 representing a high level of aggressiveness among the strains studied; the percent of pathogenicity has been affected to 92% after 11 days of the in vitro inoculation and 100% after 20 days of the in vivo inoculation
Summary
Strawberry plant, Fragaria x ananassa, is one of the most widespread horticultural crop in the world. Ten years later the culture extended to the perimeter of the Gharb and the area of Souss [1] The climate in these regions allows the growth of strawberry in greenhouses but commercial fields are ideal for the development of a large number of diseases like anthracnose. The latter is regarded as an economically important disease affecting different hosts in the world caused by different species of Colletotrichum [2] [3]. Colletotrichum acutatum is the most frequently species reported of the genus It is today known as especially destructive on strawberry fruit [8]. The aim of this study was to examine the behavior of seven isolates of Colletotrichum acutatum by changing the medium composition, temperature, pH and water activity and to study the disease severity caused by these isolates on Fragaria x ananassa in vitro and in vivo
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
