Abstract
The main objective of this work was to assess the cytotoxic activity of Au/Pt/ZnO nanoparticles synthesized using Arctium lappa extract against leukemia. The Au/Pt/ZnO nanoparticles obtained as a result of biological synthesis were characterized by UV-Vis, Scanning (SEM) and Transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), and Atomic Force Microscopy (AFM). The applied methods showed that the size of nanoparticles ranged from 10 to 40 nm. This work also assessed the cytotoxicity of Au/Pt/ZnO nanoparticles by means of MTT assay, and analyzed apoptosis as well as the influence of the cultivation time and concentration of Au/Pt/ZnO nanoparticles on the percentage of dead cells. The studies showed that the percentage of dead leukemia cells increased with the cultivation time and concentration of Au/Pt/ZnO nanoparticles. There was observed an increase in the percentage of cells in the G2/M phase, which suggests the stoppage of G2/M leading to cell death. The cytotoxicity of Au/Pt/ZnO nanoparticles determined by means of the MTT test indicated that the viability of leukemia cells practically disappeared when the concentration of the tested nanoparticles was 10 mol.
Highlights
Metal nanoparticles obtained from nanotechnology have gained global interest because of their broad applicability in biomedical and physiochemical fields
Due to the characteristics of biologically active compounds, Au/Pt/ZnO nanoparticles were obtained from A. lappa extract, and they were assessed in terms of their effects on leukemia
The UV-absorption spectrum of the Au/Pt/ZnO nanoparticles biosynthesized using A.lappa extract was monitored after continuous stirring for 12 h and 24 h of reaction at 75 °C
Summary
Metal nanoparticles obtained from nanotechnology have gained global interest because of their broad applicability in biomedical and physiochemical fields. The world literature depicts various physical and chemical methods for producing trimetallic nanoparticles. The majority of those methods are expensive and create a potential risk to the environment and organisms. In this decade, the focus on obtaining eco-friendly products in eco-friendly ways is increasing, and we are trying to find alternative solutions (Hasan et al 2018). One example of that is the biological synthesis of metal nanoparticles. The eco-friendly production of nanoparticles is possible with the use of biological
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