Abstract
The accumulation of hydrocarbon waste, such as used engine oils in environments, has many impacts on humans and other organisms, therefore many researches were achieved to degrade or remove or consume these pollutants. The aim of the current study is to get a local bacterial isolates has high ability to degrade the spent engine oil as a single or mixed culture. Five soil samples contaminated with spent engine oil were collected from mechanic workshops in Baghdad city to isolate degrading bacteria using Bushnell Hans medium (BHM), pH 7 with 5% of used engine oil. While the growth patterns and gravimetric analysis was used to reveal the ability of these isolates to degrade spent engine oil in liquid BHM medium. The best three isolates A4, B6 and D5 were identified and the optimal temperature and pH for biodegradation of spending engine oil were studied. Also, the consortium culture of three isolates was tested their ability to utilize spent engine oil under the same conditions for single isolate. Twenty five bacterial isolates were obtained from contaminated soil samples and three isolates appeared a maximum degradation rate 74.6, 70.2 and 78.5% respectively. The results from identification tests were showing these isolates belong to Bacillus sp., Acinetobacter sp. and Pseudomonas sp., respectively. The studied three isolates gave the best degradation when incubated at 30°C in BHM medium pH 7. While other results were indicated that consortium cultures are more effective 90.2% than all experiments that used single isolate.
Highlights
سَج اىَحزمبث اىَظخهيل, عشلاث بنخُزَت, ٍ اىخحيو اىبُىىىج: اىنيَبث اىذاىت Introduction Pollution with hydrocarbon materials and its products such as gasoline, fuel, engine oil, residues of fuel, and spent engine oil, has more attention according to their effects on environment pollution [1]
Five soil samples contaminated with spent engine oil were collected from mechanic workshops in Baghdad city to isolate degrading bacteria using Bushnell Hans medium (BHM), pH 7 with 5% of used engine oil
The aims of this study were focused on the isolation and screening of bacteria that degrade the spent engine oil and study the effect of temperature and pH on degradation of used oil, the effect of single and mixed cultures on degradation was detected
Summary
Collection of contaminated soils Soils contaminated with mainly spent engine oil were collected from five different areas of mechanic workshops in Baghdad city during 1-28 April, 2016. Isolation of Bacteria The samples of used engine oil-contaminated soils were used to isolate the bacteria by ino culating 1 g from them in flasks 50 ml of liquid Bushnell Haas medium (BH medium) supplemented with 5% spent engine oil as carbon source and the flasks were incubated in shaker incubator 150 rpm at 30oC for 7 days. The flasks were incubated in shaker incubator 150 rpm at 30 ̊C for 10 days, and the bacterial growth and biodegradation of used engine oil were determined [15]. Results and discussions Bacteria isolated from samples Twenty five bacterial isolates were obtained from the five soil samples collected from mechanic workshops using the solid BH medium, pH 7 supplemented with 5% spent engine oil as carbon source. The results in Table (2) exhibited that the isolates have different capability to consume the substrate and the isolates A4, B6 and D5 appeared the highest growth rate 0.67, 0.61 and 0.73 respectively, compared with other isolates in this study
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