Biological treatment of hypersaline wastewater in a continuous two-phase partitioning bioreactor: Analysis of the response to step, ramp and impulse loadings and applicability evaluation

Abstract

Abstract The response of a continuous two-phase partitioning bioreactor (C-TPPB) operated with polymeric tubing treating hypersaline wastewater was investigated under dynamic load conditions of step, ramp and impulse inputs of the influent flow rate. Tests were performed with synthetic wastewater consisting of NaCl (100 g L−1) and 2,4-dimethylphenol (DMP) (∼1200 mg L−1) to simulate the organic fraction. A biomass specifically acclimatized to the compound was utilized in the tests. The experimental system provides separation of the toxic wastewater flowing inside the polymeric tubing (coiled in the bioreactor) from the microbial culture present in the bulk bioreactor phase with the polymer providing permeability to the organic molecules as well as a barrier to salt transport. These features allowed achieving high performance even in the most severe loading conditions. Removal efficiencies >96% were obtained for DMP under all investigated load conditions (i.e. for influent salt and organic loads up to six times the base case load). A DMP mass balance at the end of the dynamic tests showed that 88% of the removed DMP was biodegraded and only 8% was retained into the polymer tubing itself. No significant variation of the DMP concentration in the bioreactor was observed in all cases thus demonstrating the complete removal of the transferred substrate and the effective performance of the biomass, which was not affected by the applied dynamic loads. A comparative analysis of C-TPPB results with the performance data of the classical technologies commonly applied for saline wastewater treatment has been performed to evaluate the system applicability.