Abstract
Objectives: Tuberous root extract based synthesis of silver nanoparticles (AgNPs), characterizations using Fourier-transform infrared spectroscopy (FT-IR), UV-visible, powder X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), energy dispersive X-ray (EDX) techniques, and antibacterial activity of AgNPs against Staphylococcus aureus, Staphylococcus mutans, Proteus vulgaris, and Escherichia coli.
 Methods: Root extract of Ipomoea batatas (sweet potato) was prepared by boiling of small cut pieces of root with double distilled water. Added root extract with silver nitrate solution and centrifuged and collect the pellets. After several washing and drying, AgNPs have been preserved for characterizations and antibacterial activity.
 Results: The synthesized AgNPs were well characterized by FT-IR, UV-visible, XRD, FESEM, and EDX methods, and significant zones of inhibition observed around the loaded AgNPs on the agar plates. The zones of inhibition have been achieved 36, 40, 46, and 32 mm for E. coli, P. vulgaris, S. mutans, and S. aureus.
 Conclusion: The characterisation methods such as UV-Visible, FTIR, Powder XRD, FESEM and EDX indicate an efficient formation of AgNPs using root extract of I. batatas. The biologically synthesized AgNPs are found good antibacterial agents.
Highlights
Nanotechnology in chemical, biological, and pharmaceutical sciences is the practice of production, working, and applications of materials under nano ranges (1–100 nm)
After synthesis of nanoparticles using any biomaterials, suitable characterization methods are required to explore the physicochemical properties of nanoparticles
All the chemicals used in experimental work were of analytical grade (AR) and the glassware washed with 10% HNO3 and double distilled water and dried in tray dryer
Summary
Nanotechnology in chemical, biological, and pharmaceutical sciences is the practice of production, working, and applications of materials under nano ranges (1–100 nm). A green synthetic process has many advantages over the conventional methods such as very low cost, minimization of harmful chemicals, eco-friendly, no additional cost on instrumentations, and highly efficient Metal nanoparticles such as gold (Au), silver (Ag), palladium (Pd), copper (Cu), and platinum (Pt) have been synthesized extensively due to their importance in pharmaceutical and other industries [2,3,4]. These metal nanoparticles exhibited very unique physical and chemical properties and are useful in electronics, photonics, catalysis, and biomedical fields [5,6,7,8]. It is necessary to all health-care industries to characterize nanomaterials using the abovementioned techniques before applications [11,12,13]
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