Abstract

The continuous pig kidney cell line (PS) is very sensitive to the propagation of Mokola virus. No prior adaptation of the virus to the cells was necessary before the demonstration of high virus titers. Virus activity in the cells can be easily quantified in terms of cytopathic effect, titer of complement-fixing antigen in the culture fluid, and plaque formation. There was no inclusion body formation. The plaques were quite clear, measuring 1–2 mm, and were countable from the 4th day. The results of the plaque neutralization test as well as the neutralization test in baby mice showed no significant cross-reaction between Mokola virus and rabies virus.

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