Biological screening of Ajuga iva extracts obtained by supercritical carbon dioxide and pressurized liquid extraction

Mohamed Djamel Miara,Hamdi Bendif,Mohamed Lazali,Petras Rimantas Venskutonis,Messaoud Boudjeniba,Mohammed Harir

doi:10.25081/jmb.2017.v1.816

Abstract

Extracts obtained by supercritical carbon dioxide extraction (SCE) and pressurized liquid extraction (PLE) from the Ajuga iva aerial parts growth in three different geographical sites of Alegria were analysed. Tocopherols in SCE Extracts were quantified by HPLC, α-tocopherol dominated the profile in both A. iva of Bejaia (BEJ) and Bordj Bou Arreridj (BBA), while β-tocopherol was the lowest. PLE method produced higher extraction yields than SCE method, which ranged from 0.31 to 24.95% (w/w), the water gave the best yields extraction. Total phenolic content (TPC) ranged from 15.60 to 63.45mg GAE/g DWE; also the water gave higher total yield of phenolics, and the best TPC from DWP. The antioxidant activity was assessed by different assays, water and SCE extracts showed a slightly higher antioxidative effect than that obtained by Ethanol and Acetone. A slight opposite trend was obtained with the FRAP antioxidant activity; Acetone was the best extract after SCE extracts. Small differences in antioxidant activity values were found between the different sites of A. iva: BEJ, BBA, and ELO.

Highlights

The genus Ajuga (Lamiaceae) comprises 40-50 species, which grow in different parts of the world (Tsurng-Juhn et al, 2009), mainly in the temperate and warm temperate zones (Yusuke et al, 2013)
A. iva is a common species in North Africa (Ayari et al, 2013), which is widely distributed in the Mediterranean region, in Algeria, Morocco, Tunisia, and Egypt, southern Europe and northern Africa
Samples preparation and extraction Mature aerial parts of A. iva were collected between April and May 2013, from the sites of Bejaia (BEJ), Bordj Bou Arreridj (BBA), El Oued (ELO) (Table 1), with difrferent climatic conditions

Summary

Introduction

50 species, which grow in different parts of the world (Tsurng-Juhn et al, 2009), mainly in the temperate and warm temperate zones (Yusuke et al, 2013). Ajuga plants are evergreen, clumpforming rhizomatous perennial or annual herbaceous flowering species, 5-50 cm tall, with opposite leaves. The flowers are two lipped and tubular, and mostly blue, purple or yellow in color. Many Ajuga plants are used in horticulture as groundcover or border, and in rock gardens, but some are regarded as weeds. Some Ajuga species have a large number of varieties (Zafar and Badiâa, 2009). A. iva is a common species in North Africa (Ayari et al, 2013), which is widely distributed in the Mediterranean region, in Algeria, Morocco, Tunisia, and Egypt, southern Europe and northern Africa

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Results

Conclusion