Abstract
The analytical method of ring D-conjugated estrogens, the existence of which in pregnancy urine is known or estimated, was established by using HPLC. Eleven kinds of these conjugates were satisfactorily separated by using octadodecylsilica as the stationary phase and a mixture (60 : 40) of 0.5% NH4H2PO4 (pH 3.0) and methanol as the mobile phase and detected sensitively by using electrochemical detector (ECD). Conjugates having a catechol or guaiacol structure could be separately determined from those, having a phenolic structure by controlling the detector voltage. The detection limit (S/N=3) of the catechol, guaiacol and phenolic conjugates were about 2, 100 and 50 pg, respectively.
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