Abstract
Adsorption and growth of VHS virus of trout were studied in cultures of RTG-2 and FHM cells. Within 1 h virus infectivity in the culture fluid decreased by 90%. No measurable adsorption took place after this time. A higher adsorption rate was achieved by pretreatment of cell cultures with 10 μg zinc sulphate and 10 or 50 μg diethyl-aminoethyl (DEAE) dextran. In both cell lines small amounts of viral antigen could be detected in the perinuclear region by the immunofluorescence technique as early as 3 h after inoculation. Infectivity titres of the culture supernatants started to rise 8–10 h post-infection and reached their maximum after 18 h. In both cell lines, 50% or more of the total infectivity remained cell associated, the yields of free virus obtained from FHM cells being 0.5 log 10 TCID 50 lower.
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