Abstract
An ethanol extract prepared from the gametophyte Chilean native moss Sphagnum magellanicum was dried out, weighed and dissolved in distilled water. This extract was then assayed for its antibacterial activity against the G(-) bacteria Azotobacter vinelandii, Erwinia carotovora subsp. carotovora, Enterobacter aerogenes, Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi, Vibrio cholerae, and the G(+) bacteria Staphylococcus aureus subsp. aureus, and Streptococcus type beta. The growth of the cultures of E. carotovora subsp. carotovora, and V. cholerae was inhibited at a concentration of 581 microg/ml of extract, while the cultures of E. coli, S. typhi and Streptococcus type beta were inhibited at a concentration of 1.16 microg/mL of extract. The concentration of phenolic compounds was 4.294 mg/mL; the presence of vanillic, chlorogenic, syringic, caffeic, gallic, 3-4 hydrozybenzoic, p-coumaric and salicylic acids was identified using RP- High Pressure Liquid Chromatography.
Highlights
The moss species, Sphagnum magellanicum Brid. (Sphagnaceae), in its natural form, is found in Chile from parallels 37o 34’ 58.88”S to 44o 02’ 11.90”S South Latitude
This extract was assayed for its antibacterial activity against the G(-) bacteria Azotobacter vinelandii, Erwinia carotovora subsp. carotovora, Enterobacter aerogenes, Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi, Vibrio cholerae, and the G(+) bacteria Staphylococcus aureus subsp. aureus, and Streptococcus type β
In relation to these studies, the objective of the present investigation was to study the effects of ethanol extracts of Chilean native moss S. magellanicum on the in vitro growth of the G(-) bacteria: Azotobacter vinelandii, Erwinia carotovora subsp. carotovora, Enterobacter aerogenes, Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi, Vibrio cholerae, and the G(+) bacteria: Staphylococcus aureus subsp. aureus and Streptococcus type β
Summary
The moss species, Sphagnum magellanicum Brid. (Sphagnaceae), in its natural form, is found in Chile from parallels 37o 34’ 58.88”S to 44o 02’ 11.90”S South Latitude. The antimicrobial properties of the ethanol extract have been studied in various species of Sphagnum (Basile et al, 1999) and in the Indian native moss Sphagnum junghuhnianum (Singh et al., 2006). In relation to these studies, the objective of the present investigation was to study the effects of ethanol extracts of Chilean native moss S. magellanicum on the in vitro growth of the G(-) bacteria: Azotobacter vinelandii, Erwinia carotovora subsp. The phenolic compounds present in the ethanol extract, which may have antioxidant capacity, were studied
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