Abstract
A pair ofYersinia enterocolitica serotype O∶3 strains — incubated in growth medium with 10% NaCl and 2 mM glycin betaine at 4 °C, were used to study the plasmid role in the infection of BALB/c mice. The isogenic plasmid-bearing strain, but not its plasmidless derivative, caused enteric infection and histological changes in intestines, stomach and liver of the mice. Two strainsY. enterocolitica andYersinia pseudotuberculosis were incubated at different temperatures (4 and 25 °C) in media, supplemented with different concentrations of NaCl. Two concentrations of betaine as osmoprotector were tested. The initial strains and their substrains were characterised by protein profiles using sodium dodecyl sulphate-polyacrylamide gel electrophoresis. Results show that in salinity conditions, the presence of osmoprotectant (betaine) as well as the temperature of cultivation plays a great role in the expression of some bacterial proteins. The manner in which the different strains answer to the stress situations is specific for each of them.
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