Biological Microarray for Detection of Somatic Mutations in the Genes of Isocitrate Dehydrogenase 1 and 2

Abstract

Mutations in isocitrate dehydrogenases 1 and 2 (IDH1 and IDH2) genes are important diagnostic and prognostic markers in a number of tumors (chondroid tumors, brain tumors, leukemia). To determine mutations in the IDH1/2 genes the method of allele-specific hybridization on a biological microarray was developed, which makes it possible to determine the presence of a mutation in a tumor sample (chondroid tumors and glioblastoma) and the variant of nucleotide substitution. When comparing IDH1/2 mutation detection methods using a biological microarray and DNA melting analysis with a TaqMan probe, full coincidence of results was obtained, with the biochip method making it possible to determine the type of nucleotide substitution without an additional sequencing step.