Abstract
Experiments were performed to determine whether bacterial inactivation in the separated-surface-sensitizer system for singlet oxygen generation is due to O 2( 1 Δ g) or O 2( 1 Σ g +). The rates of inactivation of Gram-negative Salmonella typhimurium LT-2 and a nonpigmented strain of Gram-positive Sarcina lutea were found linearly with the concentration of 1 Δ g. The gas phase lifetime of the inactivating agent was found to be within the range of values expected for the gas phase lifetime of 1 Δ g rather than 1 Σ g +. These measurements conclusively demonstrate that bacterial inactivation in this system is due predominantly to 1 Δ g. Therefore, studies of bacterial inactivation with this singlet oxygen generating system can be used to assess the role of singlet oxygen in various biological and medically relevant situations.
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Schedule a callMore From: Biochimica et Biophysica Acta (BBA) - General Subjects
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