Abstract
Colorectal cancer is one of the leading death-related diseases worldwide, usually induced by a multifactorial and complex process, including genetic and epigenetic abnormalities and the impact of diet and lifestyle. In the present study, we evaluated the biological impact of two of the main coffee polyphenols, chlorogenic acid (CGA) and caffeic acid (CA), as well as two polyphenol-rich coffee extracts (green coffee extract and toasted coffee Extract) against SW480 and SW620 colorectal cancer cells. First, the total phenolic content and the antioxidant capability of the extracts were determined. Then, cytotoxicity was evaluated by MTT and SBR. Finally, a wound healing assay was performed to determine the impact on the cell migration process. The results showed a cytotoxic effect of all treatments in a time and dose-dependent manner, which decreased the viability in both cell lines at 24 h and 48 h; likewise, the migration capability of cells decreased with low doses of treatments. These results suggest the potential of coffee to modulate biological mechanisms involved in colorectal cancer development; however, more studies are required to understand the mechanistic insights of these observations.
Highlights
Colorectal cancer (CRC) is one of the most common and deadly diseases globally, with1.9 million new cases and approximately 935,000 deaths in 2020 [1]
In the present in the present study, we evaluated in tumor‐derived CRC cell lines, the biological activity study, we evaluated in tumor-derived CRC cell lines, the biological activity of two coffee of two coffee extracts from Coffee Arabica collected in the Andean extracts from Coffee Arabica collected in the Andean mountains of mountains of Antioquia‐Colombia and two polyphenols (CA and chlorogenic acid (CGA)) present in coffee
Our study shows that the migratory capabilities of SW480 and SW620 without treatments and SBF supplementation were similar, with a constant velocity of wound healing and a partial closure of wound at 120 h (Figure 5)
Summary
Colorectal cancer (CRC) is one of the most common and deadly diseases globally, with1.9 million new cases and approximately 935,000 deaths in 2020 [1]. Incidence rates of CRC fluctuate worldwide, with the highest incidences found mainly in developed countries such as New Zealand, Australia, and the United States. Countries with lower CRC incidences are found in Africa and Southwest Asia. This variation in incidence could be explained by differences in eating and cultural habits between regions [2]. The stage at which cancer disease is detected determines the prognosis, survival, and patient treatment. Tumor invasion and metastasis in the middle and late stages of the disease are recognized as the leading causes of treatment failure and poor therapeutic efficacy [7,8]
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