Abstract

Biology provides the most sophisticated nanostructures in terms of components and principles of self-assembly. Near-field optical microscopy is a method for nanoscale imaging of heterogeneous biological materials under ambient or aqueous conditions. This chapter reviews its applications and discusses principles and results from the two primary types of near-field microscopy: apertureless and apertured. Examples of studies of proteins, DNA, and lipids, as well as their aggregates, and cells and their organelles are presented. Instrumental and theoretical details are summarized when necessary.

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