Biological Extraction, HPLC Quantification and Medical Applications of Astaxanthin Extracted from Crawfish "Procambarus clarkii" Exoskeleton By-Product.

Abstract

Simple SummaryThe study aims to provide an eco-friendly method for the extraction of the natural pigment called “astaxanthin” from crawfish powder. Bacterial and fungal strains that are beneficial to humans were used as an alternative method of extraction instead of using chemicals. Astaxanthin concentration was determined using an analytical tool referred to as “high-performance liquid chromatography” (HPLC). The results were promising; however, future studies can provide more effective methods for obtaining better results. Several tests were done to evaluate the biological activity of extracted astaxanthin such as antifungal, anti-inflammatory, antioxidant, and anticancer. Further purification of the extracted astaxanthin from crawfish exoskeleton needed to be done to assure that the results of the tests obtained were only due to the action of astaxanthin.The main challenge of astaxanthin extraction is to provide an eco-friendly method of extraction instead of chemical methods that harm human health. This study provided an eco-friendly method for astaxanthin extraction using two bacterial and fungal probiotics (Bifidobacterium lactis, Lactobacillus lactis, Candida utilis, and Saccharomyces cerevisiae, respectively) and determined the astaxanthin concentration by high-performance liquid chromatography (HPLC) analysis. The results showed that the highest concentration was obtained by S. cerevisiae (45.69 µg/g). Several biological tests were done on the exoskeleton containing astaxanthin of crawfish. Antifungal activity was effective against C. utilis (inhibition zone is 12.3 ± 0.5 mm). The scavenging percentage of 2,2-diphenyl-1-picrylhydrazyl (DPPH scavenging percentage) was 72.1% at 1000 µg/mL concentration of exoskeleton containing astaxanthin. The Hemolysis inhibition percentage was 65% at the same concentration used previously. Furthermore, the IC50 value of human liver cancer cell line (HepG2), human hepatocellular carcinoma (HCT), and breast cancer cell line MCF-7 were 24 µg/mL, 11 µg/mL, and 9.5 µg/mL, respectively. The least cell viability percentage was 19% (using breast cancer cell line (MCF-7)) at 100 µg/mL of exoskeleton containing astaxanthin. Thus, using microorganisms can be an alternative and promising way of astaxanthin extraction. Furthermore, purification of extracted astaxanthin is essential for medical applications.