Biological evaluation of hesperidin for direct pulp capping in dogs' teeth.

Abstract

This study compared the biological effect of Hesperidin, Mineral Trioxide Aggregate (MTA)-Angelus and calcium hydroxide for direct pulp capping. A total of 126 dogs, teeth were divided according to the post-treatment evaluation period into three groups (42 teeth each), group I: 2weeks, group II: 4weeks and group III: 8weeks. Each group was further subdivided according to the pulp capping material into three subgroups (14 teeth each), subgroup A (Hesperidin), subgroup B (MTA-Angelus) and subgroup C (Dycal). Both inflammatory response and dentine bridge formation were assessed by histopathology. All data were statistically analysed. Resolution of the inflammation was recorded by the time with a significant difference between subgroups within the same group (P<.05). Hesperidin, MTA-Angelus and Dycal showed either mild or moderate inflammation at 2weeks with significant differences between subgroups (P<.05). At 4 and 8weeks, there were no significant differences between subgroups (P>.05). Absence of complete or partial calcified bridge with no odontoblastic layer was reported in all subgroups at 2weeks while at 4weeks, the majority of samples in Hesperidin and MTA subgroups showed amorphous calcified deposit. At 8weeks, there was no significant difference (P>.05) between subgroups except that 78.5% and 92.9% of Hesperidin and MTA-Angelus samples, respectively, showed moderate dentine bridge. Also, 78.5% of Hesperidin and Dycal samples revealed moderately thick dentine bridge while 78.7% of MTA-Angelus showed a thin dentine bridge with a significant difference between them (P<.05). In conclusion, Hesperidin is a promising pulp capping material inducing mild inflammation and good dentine bridge formation.