Abstract
Shikonin, an active ingredient of Lithospermum erythrorhizon, exerts anti-inflammatory and antibacterial effects, and promotes wound healing. We investigated whether shikonin stimulated gingival tissue wound healing in human gingival fibroblasts (hGF). In addition, we evaluated the effects of shikonin on the mitogen-activated protein kinase (MAPK) signaling pathway, which has an important role in wound healing. hGF were subjected to primary culture using gingiva collected from patients. The cells were exposed to/treated with Shikonin at concentrations ranging from 0.01 to 100 μM. The optimal concentration was determined by cell proliferation and migration assays. Type I collagen and fibronectin synthesis, the gene expression of vascular endothelial growth factor (VEGF) and FN, and the phosphorylation of Extracellular signal-regulated kinase (ERK) 1/2 were investigated. Identical experiments were performed in the presence of PD98059 our data suggest, a specific ERK 1/2 inhibitor. Shikonin significantly promoted hGF proliferation and migration. Shikonin (1 µM) was chosen as the optimal concentration. Shikonin promoted type I collagen and FN synthesis, increased VEGF and FN expression, and induced ERK 1/2 phosphorylation. These changes were partially suppressed by PD98059. In conclusion, Shikonin promoted the proliferation, migration, type I collagen and FN synthesis, and expression of VEGF and FN via ERK 1/2 signaling pathway in hGFs. Therefore, shikonin may promote periodontal tissue wound healing.
Highlights
Periodontal surgery is a common treatment for periodontal disease, and healing of the soft tissue is critical for the success of this therapeutic approach
Pathway in hGFsproliferation and migration of human gingival fibroblasts (hGF), we investigate mechanism of the shikonin-induced evaluated the activation of Extracellular signal-regulated kinase (ERK) 1/2
We found that shikonin activated the ERK 1/2 signaling pathway in hGF
Summary
Periodontal surgery is a common treatment for periodontal disease, and healing of the soft tissue is critical for the success of this therapeutic approach. Human gingival fibroblasts (hGFs) play an important role in the cell growth and remodeling phases of oral wound healing in gingival tissue [3,4,5,6,7,8]. Derived substances, including herbs, have been used as medicines for curing wounds for millennia, and have been studied extensively for their healing properties [9,10,11,12,13]. The natural substances acemannan has been used to treat burns and skin wounds. We decided to investigate the ability of the purple roots of Lithospermum erythrorhizon, a natural substance, to promote wound healing. The roots of L. erythrorhizon have been used to treat wounds, burns, ulcers, and other conditions since ancient times [15]. Shikonin promotes wound healing [16,17], and exerts anti-inflammatory [18,19], antitumor [20,21], and antimicrobial effects [22]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
