Biological Effects of Osteoblast-Like Cells on Nanohydroxyapatite Particles at a Low Concentration Range

Xiaochen Liu,Shicheng Wei,Jie Wei

doi:10.1155/2011/104747

Abstract

The biological effects of osteoblast-like MG-63 cells on nanohydroxyapatite (n-HA) at the low concentration range (5–25 g/mL) for 5 days was investigated. The results showed the viability and actin cytoskeleton of the cells descended with the increase of the concentration of n-HA, and the actin cytoskeleton of cells was depolymerised and became more disordered. Apoptotic rate of cells (1.85%, 1.99%, and 2.29%) increased with the increase of n-HA concentration (5, 15, and 25 g/mL) and become significantly higher than the control. Total intracellular protein content decreased with n-HA concentration increase, showing significant difference between 25 g/mL and the control, and no significant change of ALP activity was observed at the 5th day. The results revealed that the cell growth was inhibited by n-HA in a concentration-dependent manner, and the obvious biological effects of MG-63 cells on n-HA existed at the low concentration range from 5 to 25 g/mL.

Highlights

Hydroxyapatite (HA), with the structural formula of Ca10(PO4)6(OH)2, is the principal inorganic constituent of human bone [1]
The results demonstrated that both cell proliferation and cell apoptosis were related to the concentration of nHA
At the low concentration range from 5 to 25 μg/mL, the n-HA inhibited the proliferation of MG-63 cells, and total intracellular protein content decreased with increase of the n-HA concentration

Summary

Introduction

Hydroxyapatite (HA), with the structural formula of Ca10(PO4)6(OH), is the principal inorganic constituent of human bone [1]. It is imperative to be assessed, they are even used in clinic with a very small amount or a relatively low concentration at the local region. HA or n-HA coating implants had been proven to produce small amounts of particulate debris (52 ± 51 particles per mm2) in acetabular components from patients with osteolysis [12], in which the concentration is apparently lower than that mentioned above. Osteoblasts are selected since these cells stay in direct contact with the implanted materials during the bone growth stage. If osteoblasts die on the implanted materials surface because of the presence of debris, the new bone will not be generated. The aim of this study was to investigate the responses of MG-63 cells cultured with n-HA slurry at a relatively low concentration range and to evaluate

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Conclusion