Abstract
A low velocity (0–1000 V) electron gun for use in biological and biochemical research is described. Results of experiments on the bombardment of bacteria and yeast with electrons accelerated by potentials ranging from 0 to 750 V show that these organisms are killed at threshold electron velocities. Staphylococcus albus, which has an average size of 0.7–0.9 μ, requires electron accelerating potentials of approximately 25 V and over to produce a lethal effect, while Saccharomyces cerevisiae, with an average diameter of 50 μ, is killed only by electrons which have been accelerated by potentials in excess of 300 V, for the entire energy range tested. The experiments also establish the fact that the energy required to kill yeast cells is approximately 12 times that required to kill S. albus.
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