Abstract
In order to study the mechanism of high-voltage electric field (HVEF) biotechnology, corona discharge produced by a multi-needle-plate HVEF was used to treat naked oat seeds, each treatment dose was divided into two groups, one group was covered with a petri dish cover, the other group was directly exposed to HVEF without a petri dish cover. The scanning electron microscope (SEM) results show that the etching degree of the uncovered group was more serious than that of the covered group, it indicates that ion wind etching has a greater impact on the micro-morphology of seed coat, being covered can effectively reduce the etching degree of discharge plasma on seed. Fourier Transform infrared spectroscopy (FTIR) of the seed coat shows most of the HVEF treatment group can form a new absorption peak at 1740 cm−1, which is closely related to the hydrophilicity of the seed. Comprehensive analysis shows that HVEF treatment can improve the hydrophilicity of seeds, whether they are covered or not. Being covered can reduce the degree of etching of the seed coat, but increase the hydrophilicity of the seed, indicating that the non-uniform electric field has a greater impact on the hydrophilicity of the seed. Our study showed that ion wind had an effect on the micro-morphology of seeds, but this effect didn’t translate into a macroscopic effect. This study provides ideas and experimental data support for the study of the biotechnological mechanism of HVEF.
Highlights
The naked oat is a kind of grain-grass-type crop that is cold-loving, drought-resistant and with a strong barren resistance, and it has the highest protein content in cereal grains and is a health-care grain crop [1]
Except for the 8 kV uncovered treatment group, the water absorption of the other treatment groups was improved by different degrees compared to the control group, and the water absorption rate of the covered group increased under each voltage
When there is no cover, multi-factor synergy has a greater influence on the microstructure of the seed coat, indicating that the ion wind etch has a greater influence on the micro-morphology of the seed coat than the non-uniform electric field, but this effect is not converted into a macroscopic effect
Summary
The naked oat is a kind of grain-grass-type crop that is cold-loving, drought-resistant and with a strong barren resistance, and it has the highest protein content in cereal grains and is a health-care grain crop [1]. It plays an important role in improving people’s diet and ecological construction of agriculture and animal husbandry. China’s main naked oat producing areas are combined with semi-agricultural and semi-pastoral areas to breed drought-resistant, barren-resistant, high-yield and high-quality varieties This is more important as a new naked oat variety is being used for both grain and grass. It is very important to study the biological effects of high-voltage electric field (HVEF) on naked oat seeds to improve the quality and yield of naked oat
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