Abstract
Biological effects of external polysaccharide (EPS) isolated from Sinorhizobium meliloti bacteria on tissue culture of Fenugreek plant
Highlights
External Polysacchariede has been extracted from Sinorhizobium meliloti which is known for its symbiotic relation with Legume plant Trigonella foenum-graecum
It has been shown that all these concentrations did not have any effect on callus induction from the roots of this plant
While the concentration (0.1) mg/l.; was the best one that stimulated callus formation from stems and leaves explants since the percentage of callus induction were 50 and 62.5% respectively. This concentration of EPS was added to the treatments that contain both auxin and cytokinin in Murashige & Skoog (MS) medium.the results showed thata a white callus was formed from stems and leaves explants after two weeks of culture on solidified MS medium containing 0.1 mg /L of both EPS and NAA with differentiation of roots from leaves' callus
Summary
External Polysacchariede has been extracted from Sinorhizobium meliloti which is known for its symbiotic relation with Legume plant Trigonella foenum-graecum.
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