Abstract
Among people that might take a large amount of sucralose, are diabetic people who are attempting to modify their carbohydrate intake. The objective of this study is to evaluate the impact of sucralose; an artificial sweetener derived from sucrose, at a dose approximately twice the ADI on hyperglycemia, hyperlipidemia and oxidative stress in diabetic rats. Diabetes was induced in male albino rats after an intraperitoneal streptozotocin injection (65 mg/kg body weight). Animals with fasting blood glucose levels ≥250 mg/dl were considered diabetics. Sucralose was dissolved in water and administered to rats daily by oral gavages during a period of 6 weeks at a dose of 11 mg/kg body weight. Animals were divided into 4 groups and treated in parallel for 6 weeks. Control: rats received distilled water, Sucralose: rats received sucralose, Diabetic: diabetic rats received distilled water, Diabeticrats + Sucralose: diabetic rats received sucralose. The administration of sucralose to diabetic rats provoked a significant decrease (P P P > 0.05) on insulin, compared to their respective values in diabetic rats receiving distilled water. Biochemical analysis in brain and testis tissues showed that sucralose has no effect (P > 0.05) on superoxide dismutase (SOD), catalase, glutathione peroxidase (GSH-Px), and glucose-6-phosphate dehydrogenase (G-6-PDH) activities, and glutathione content (GSH), while reduced thiobarbituric acid reactive substances level (TBARS) (P < 0.05), compared to their respective values in diabetic rats receiving distilled water. It could be concluded that consumption of sucralose didn’t induce oxidative stress, has no effect on insulin, reduce glucose absorption and intensify hypercholesterolemia in STZ-induced diabetic rats. Accordingly it is advised that diabetic people consuming high amount of sucralose must check their lipid profile to avoid diabetic complications.
Highlights
Diabetes mellitus, or diabetes, is a metabolic disease characterized by chronic hyperglycemia
Biochemical analysis in brain and testis tissues showed that sucralose has no effect (P > 0.05) on superoxide dismutase (SOD), catalase, glutathione peroxidase (GSH-Px), and glucose-6-phosphate dehydrogenase (G-6-PDH) activities, and glutathione content (GSH), while reduced thiobarbituric acid reactive substances level (TBARS) (P < 0.05), compared to their respective values in diabetic rats receiving distilled water
The objective of this study is to evaluate the impact of sucralose at a dose approximately twice the ADI on oxidative stress in the brain and testis of STZ-induced diabetic rats, besides its effect on serum glucose and insulin levels and lipid profile
Summary
Diabetes, is a metabolic disease characterized by chronic hyperglycemia. In its enediol form, glucose is oxidized in a transition-metal dependent reaction to an enediol radical anion that is converted into reactive ketoaldehydes and to superoxide anion radicals that undergo dismutation to hydrogen peroxide. Hydrogen peroxide (H2O2) in the presence of transition metals, can lead to production of extremely reactive hydroxyl radicals. Brownlee [4] demonstrated that hyperglycemia-induced generation of superoxide anion radicals at the mitochondrial level is the initial trigger of vicious cycle of oxidative stress in diabetes. Another important source of free radicals in diabetes is the interaction of glucose with proteins leading to the forma-
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