Abstract
The aim of this study was to assess the biological effect of organically coatedGrias neuberthii (piton)fruit andPersea americana (avocado)leaves nanoparticles (NPs) on cervical cancer (HeLa) and breast adenocarcinoma (MCF-7) cells with an emphasis on gene expression (p53 transcription factor and glutathione-S-transferaseGST) and cell viability. UV-Vis spectroscopy analysis showed that synthesized AgNPs remained partially stable under cell culture conditions. HeLa cells remained viable when exposed to piton and avocado AgNPs. A statistically significant, dose-dependent cytotoxic response to both AgNPs was found on the breast cancer (MCF-7) cell line at concentrations above 50 µM. While expression levels of transcription factor p53 showed downregulation in treated MCF-7 and HeLa cells,GSTexpression was not affected in both cell lines treated. Cell viability assays along with gene expression levels in treated MCF-7 cells support a cancer cell population undergoing cell cycle arrest. The selective toxicity of biosynthesized piton/avocado AgNPs on MCF-7 cells might be of value for novel therapeutics.
Highlights
Breast and cervical cancers are the most common malignancies among females in low- and middle-income countries (LMICs). e two malignancies are associated with high mortality rates and represent a considerable burden for public health systems [1]
Stability of biosynthesized G. neuberthii AgNPs and P. americana AgNPs in biological medium and distilled water was evaluated by UV-visible spectroscopy for 3 incubation times under standard conditions of 37°C and 5% CO2 in a humidified atmosphere (see supplementary data)
UV-Vis spectra of all samples showed a broad peak at the maximum absorption wavelength between 400 and 420 nm due to the surface plasmon resonance (SPR) band of spherical AgNPs
Summary
Breast and cervical cancers are the most common malignancies among females in low- and middle-income countries (LMICs). e two malignancies are associated with high mortality rates and represent a considerable burden for public health systems [1]. Since cancer is a world health problem, emerging drug preparations that can pass through tumor barriers and enhance anticancer drug delivery are potentially useful [2]. In this context, a lot of research has been done to synthesize new classes of materials, including those at nanoscale, and test their anticancer properties and/or their application in cancer early detection approaches [3, 4]. Silver nanoparticles (AgNPs) are widely applied in cancer research due to their potent in vitro antitumor effects on cancer cell lines including breast and cervical cancer models [5, 6].
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