Biological Differences between Five Populations of Fall Armyworm (Lepidoptera: Noctuidae) Collected from Corn in Mexico

Abstract

Biological characterizations of five fall armyworm populations, Spodoptera frugiperda (J. E. Smith) (FAW) collected from corn, Zea mays L., in Mexico, were reared and evaluated under laboratory conditions. The period from larvae to pupal stage, pupal weights, and survival rates were determined. The reproductive compatibility of adults, and the neonatal susceptibility to Endosulfan, Carbofuran and Bacillus thuringiensis (Bt) were also evaluated. Populations from Aguascalientes, Colima, Nuevo Leon, Sinaloa, and Yucatan were reared on corn at 25C. The Colima population reared on corn leaves required the least number of days to reach the pupal stage (13.04 D). Significant differences between the pupal weights of the different populations were found, ranging from 0.215 to 0.156 g. Survival rates varied from 80 to 45%, the Colima and Sinaloa populations had the highest survival. The Aguascalientes, Nuevo Leon and Yucatan populations were reproductively compatible as they produced progeny when paired. However, no progeny were obtained when the Colima and Sinaloa populations were paired with any other populations. The Aguascalientes, Nuevo Leon and Sinaloa populations tested for susceptibility to B. thuringiensis resulted in LC50 values, from 0.001 to 0.045 mg/ml). The Aguascalientes and Yucatan populations showed similar susceptibility to Carbofuran and Endosulfan insecticide with an LC50 ranging from 0.033 to 0.188 mg/ml, and 0.023 to 0.054 mg/ml, respectively. The Nuevo Leon population was the least susceptible. Results suggest that two corn FAW strains may have developed reproductive isolation due to geographic isolation. One strain formed by the Yucatan, Aguascalientes and Nuevo Leon populations, which are distributed along the Coastal Gulf and the geographic center of Mexico, and the other corn strain is formed by the Colima and Sinaloa populations found along the Mexican Pacific Coast, as the two strains produce no progeny when paired.