Abstract

Poly(butanediol succinate) (PBS), a biodegradable polymer, was used as both solid carbon source and biofilm carrier for biological nitrate removal process, in which PBS was filled in a packed-bed bioreactor. The denitrification performance and the microbial diversity of biofilm attached on the surface of PBS were investigated. The experimental results showed that the volumetric denitrification rate was 0.60kgm(-3)day(-1) when NO3-N loading rate was 0.63kgm(-3)day(-1), and the average NO2-N concentration was below 0.20mgL(-1). The effluent pH value decreased slightly from a range of 6.98-7.87 to 6.46-7.18. The analysis of microbial community structure of biofilm by pyrosequencing method showed that Proteobacteria was the most abundant phylum (89.87%), and β-Proteobacteria represented the most abundant class. Among the 76 identified genera, Dechloromonas (10.26%), Alicycliphilus (9.15%), Azospira (8.92%), and Sinobacteraceae-uncultured (8.75%) were the abundant genera. PBS, as a promising alternative carbon source, is a suitable solid carbon source and biofilm carrier for nitrate removal.

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