Biological control of Meloidogyne javanica in bean plants by Hohenbuehelia spp. and Trichoderma koningiopsis

Cleonice Lubian,Andressa Maiara Agustinha,Roberto Luis Portz,José Renato Stangarlin,Odair José Kuhn

doi:10.1590/1808-1657000772019

Abstract

ABSTRACT Nematode control strategies are limited and alternative control is demanded. The main aim was to evaluate control potential of Hohenbuehelia spp. (Hohenbuehelia mastrucata, Hohenbuehelia barbatula, Hohenbuehelia bullulifera, Hohenbuehelia portegna, Hohenbuehelia petaloides and Hohenbuehelia paraguayensis) and Trichoderma koningiopsis against Meloidogyne javanica in bean plants in greenhouse. The parameters evaluated were the number of galls and egg masses which were evaluated in three root regions: basal, intermediate and tip; nematode reproduction factor; vegetal growth parameters (plants height on V1, V2, V4, and R5 growth stages, root length, dry weight of root and aerial part dry weight); Treatments were composed by individual interactions of each fungal isolate with 4,000 eggs of M. javanica, having a total control (bean) and a partial control (bean + nematode) arranged in completely randomized design with four repetitions. Data were tested by Scott–Knott (5%). For vegetal growth parameters, there was only significance for dry weight of roots. All species were able to control nematodes.

Highlights

Meloidogyne genus, known as root gall nematode, covers polyphagous species spread all over the soils and adapted to Brazilian regions (MACHADO, 2014)
The parameters evaluated were the number of galls and egg masses which were evaluated in three root regions: basal, intermediate and tip; nematode reproduction factor; vegetal growth parameters; Treatments were composed by individual interactions of each fungal isolate with 4,000 eggs of M. javanica, having a total control and a partial control arranged in completely randomized design with four repetitions
There are over 90 species, especially Meloidogyne arenaria, Meloidogyne incognita, Meloidogyne javanica, and Meloidogyne hapla (FERRAZ, 2018)

Summary

Introduction

Meloidogyne genus, known as root gall nematode, covers polyphagous species spread all over the soils and adapted to Brazilian regions (MACHADO, 2014). There are over 90 species, especially Meloidogyne arenaria, Meloidogyne incognita, Meloidogyne javanica, and Meloidogyne hapla (FERRAZ, 2018). Galls are direct symptoms of Meloidogyne attack, apart from some cases (FERRAZ; BROWN, 2016). Galls are an expression of a feeding site, the cenocyte, a metabolic drain essential to nematode development (FERRAZ, 2018). Nematode attack leads to cenocyte formation with eventual, frequent, galls. Galls may occur early in female maturity (FERRAZ; BROWN, 2016)

Methods

Results

Conclusion