Abstract
To obtain a kind of microbial pigment with high yield and variety coloration by regulating the pigment synthesis pathway in this experiment, Lasiodiplodia theobromae was used to induce pigment secretion by prolific inducing factors (tyrosinase, Cu2+, stilbene glycoside) and pleochromatic inducing factors (L-tyrosine, L-cysteine, tricyclazole). The results showed that through single factor and compound culture, the most reasonable inducing formula was 150 ku/L tyrosinase, while tricyclazole at 10mg/L for 30days had a maximum color difference of 42.92 NBS with a reddish-brown color in 10days. The melanin content increased gradually with the extension of culture time, and the pleochromatic inducing group was up to 3.47mg/mL, higher than that of the prolific inducing group. However, the poor solubility of purified melanin with a diameter of 100-200nm was observed in conventional solvents. Through effective induction, it is expected that L. theobromae can secrete melanin stably and be widely used in printing, dyeing, electronics, and the chemical industry.
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