Abstract
Fusarium wilt of banana (FWB) is the main threatening factor for banana production worldwide. To explore bacterial biocontrol resources for FWB, the antagonistic effective strains were isolated from banana-producing areas in Yunnan Province, China. Two isolates (YN0904 and YN1419) displaying strong antagonism against Tropical Race 4 (TR4) were identified from a total of 813 strains of endophytic bacteria. TR4 inhibition rates of YN0904 and YN1419 were 79.6% and 81.3%, respectively. By looking at morphological, molecular, physiological and biochemical characteristics, YN0904 was identified as Bacillus amyloliquefaciens, while YN1419 was identified as B. subtillis. The control effects of YN0904 and YN1419 on TR4 in greenhouse experiments were 82.6% and 85.6%, respectively. Furthermore, YN0904 obviously promoted the growth of banana plantlets. In addition, biocontrol marker genes related to the biosynthesis of antibiotics synthesized and auxin key synthetase genes could be detected in YN0904. Surprisingly, the marker gene sboA could be exclusively detected in YN1419, while other marker genes were all absent. Molecular characterization results could provide a theoretical basis for expounding the biocontrol mechanisms of these two strains. We concluded that natively antagonistic strains derived from local banana plantations could provide new biological control resources for FWB.
Highlights
Banana (Musa spp.) is a globally important tropical economic and food crop, grown in more than 130 countries [1,2]
Fusarium wilt of banana (FWB) is a globally devastating disease caused by Fusarium oxysporum f. sp. cubense; Tropical Race 4 (TR4) is the most virulent race [4]
TR4 were obtained (Supplementary Table S2), which were isolated from symptomless pseudostem samples already infected with FWB and bacterial soft rot, and the sampling stage was at banana bud formation
Summary
Banana (Musa spp.) is a globally important (sub) tropical economic and food crop, grown in more than 130 countries [1,2]. China has large banana-production areas, mainly in Guangxi, Hainan, Guangdong, Fujian, Taiwan and Yunnan provinces [3]. Yunnan Province is one of the original centers of the banana and has a long history of banana cultivation. Banana production supports a key income for ethnic groups in remote areas and offers a route to alleviate poverty, playing an important economic role in regional rural society. Fusarium wilt of banana (FWB) is a globally devastating disease caused by Fusarium oxysporum f. Being a typical soil-borne disease, TR4 is very difficult to control [5,6]. Resistant breeding is usually the most effective method; it is difficult to deploy conventional crossbreeding methods due to the main commercial cultivars are triploid and seedlessness [7,8]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
