Abstract
The aim of the present research was to evaluate the antimicrobial and bio-preservative capabilities of the selected probiotic lactic acid bacteria (LAB) isolated from rice bran sourdough. The survival in the simulated stomach and intestine conditions was used to screen the LAB isolates. Subsequently, the selected LAB isolate was identified by a PCR-based assay, and then the probiotic characteristics of the isolate including auto-aggregation and co-aggregation abilities, haemolytic activity and antibiotic susceptibility profile were determined. After that, the antagonistic effects of the LAB isolate on some foodborne indicator bacteria and fungi and its anti-aflatoxigenic capability were studied. In vivo safety and in vivo anti-Listeria activity of the isolate were also investigated. Due to the results of the sequencing and phylogenetic evolutionary tree, the LAB isolate was identified as Lactobacillus brevis. The isolate had no haemolytic activity, and it showed proper antifungal effects on the tested fungi. Furthermore, the L. brevis isolate had higher (P < 0.05) co-aggregation and antibacterial activities toward L. monocytogenes rather than the other indicator bacteria. Based on the results of the HPLC analysis, anti-aflatoxigenic effect of the isolate on aflatoxin B1 was higher than the other toxins. No noticeable abnormal behaviors or changes in general health status, growth, blood biochemistry and examined liver enzymes were observed in the mice during a four-week feeding with the L. brevis in comparison with the control. The L. brevis-fed mice significantly (P < 0.05) reduced the numbers of L. monocytogenes in the gastrointestinal tract, visceral organs and faeces as well.
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