Abstract
Yeasts (52, 51, 69, and 04) were received from Biotechnology Center of Karaj and Penicillium expansum isolates P 11 and P 12 iso- lated from Golden Delicious. Isolates were evaluated as a potential biological control agents of apple blue mold caused by P. expansum. Dual culture, cell free metabolites and volatile test were used in vitro assay. All tested of yeast isolates inhibited growth of P. expansum. The inhibition varied among isolates of yeasts and ranged from 19.81% to 40.73%, in dual culture, from 43.16% to 66.44% in volatile metabolite and from 22.16% to 50.23% in cell free metabolite test. Apple fruit wounds were inoculated with 40 µl of yeast cell suspension (10 7 cell/ml) followed 48 h later by P. expansum (10 5 conidia/ ml). The apples were then incubated at 25°C. Four isolates of Saccharomyces cerevisiae reduced, the decay area from 13.46 to 24.92 cm 2
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