Biological characterization of primary and recurrent brain tumor stem cells

Abstract

Objective To investigate the biological characteristics of primary and recurrent brain tumor stem cells(BTSCs).Methods Single cell suspensions were made from the primary and recurrent brain tumor tissues,which were obtained from the Surgical specimens of the same patient.then were cultured in medium favored of neural stem cell growth.and were further passaged.frozen.resuscitated and recultured,respectively in vitro.The biological characteristics of the primary and recurrent BTSCs were further investigated.Results In serum-free medium favored for stem cell growth,both of the primary and recurrent BTSCs could form suspending soberes.In medium with serum,BTSCs attached to the bottom of culture dishes and difierentiated into polvmorphic tumor cells.Differentiation jnhibition was observed in both of BTSCs.which were heteroploid.too.Nude mice intracranial xenografi tumors of primary BTSCs exhibited invasive growth around the inoculation region:while tumors of recurrent BTSCs formed multipie disseminated foci and infiltrated the meninges widely.Conclusion Long-term maintaining of the biologically stable primary and recurrent BTSCs will lay the foundation to further investigate the mechanisms of glioma tumorigenesis,growth,invasion and dissemination. Key words: Brain tumor stem cells; Biological characteristics; Recurrence