Biological characterization and pathogenicity of three Haemonchus contortus isolates in primary infections in lambs

Abstract

The biological characterization and differential pathogenicity of three isolates of Haemonchus contortus, one autochthonous (Aran 99) and two allochthonous (Moredun Research Institute, MRI, and Merck Sharp and Dohme, MSD) were studied by primary experimental infection of Manchego lambs. Thus, six female lambs (5.5 months old) were infected with 12,000 L 3 larvae of each helminth isolate. Parasitological (pre-patent period, parasite egg shedding dynamics), biopathological (packed cell volume (PCV), haemoglobin concentration, plasma proteins, serum pepsinogen) and zootechnical parameters (live weight gain, thoracic perimeter) were measured throughout the study. After sacrifice (85 days post-infection (pi)), lamb carcasses were inspected for parasite burden and development (establishment rate, male/female ratio, degree of parasite development), and the average carcass weight of the experimental groups was compared. The autochthonous combination (Manchego lambs—Aran 99) had a longer pre-patent period (28 days) and a significantly different pattern of egg elimination (maximum elimination on day 80 pi). The establishment rate and parasite burden (average values of 8.18% and 988 adult helminths, respectively) were both low, with no significant differences between isolates. There were no significant differences in parasitic nematode development in terms of size and weight (1264.66 μm and 149.45 μg for male worms and 2093.33 μm and 411.46 μg for females, respectively), although Aran 99 females weighed less ( p < 0.05). All isolates induced a slight but significant reduction of PCV values from day 23 pi onwards. Inter-isolate differences were found, with the effects in the case of MSD being more pronounced. Variations of serum protein levels were minimal in all lamb groups. The live weight gain of MSD- and Aran 99-infected animals was significantly lower ( p < 0.05) than for MRI-infected lambs and uninfected control animals. Carcass yield from the lambs infected with the autochthonous isolate (Aran 99) was lower. The MSD isolate therefore showed a higher comparative pathogenicity.