Abstract
IntroductionBurned human skin, which is routinely excised and discarded, contains viable mesenchymal stromal/stem cells (burn-derived mesenchymal stromal/stem cells; BD-MSCs). These cells show promising potential to enable and aid wound regeneration. However, little is known about their cell characteristics and biological function.ObjectivesThis study had two aims: first, to assess critical and cellular characteristics of BD-MSCs and, second, to compare those results with multipotent well-characterized MSCs from Wharton’s jelly of human umbilical cords (umbilical cord mesenchymal stromal/stem cells, UC-MSCs).MethodsBD- and UC-MSCs were compared using immunophenotyping, multi-lineage differentiation, seahorse analysis for glycolytic and mitochondrial function, immune surface markers, and cell secretion profile assays.ResultsWhen compared to UC-MSCs, BD-MSCs demonstrated a lower mesenchymal differentiation capacity and altered inflammatory cytokine secretomes at baseline and after stimulation with lipopolysaccharides. No significant differences were found in population doubling time, colony formation, cell proliferation cell cycle, production of reactive oxygen species, glycolytic and mitochondrial function, and in the expression of major histocompatibility complex I and II and toll-like receptor (TLR).Importance, translationThis study reveals valuable insights about MSCs obtained from burned skin and show comparable cellular characteristics with UC-MSCs, highlighting their potentials in cell therapy and skin regeneration.
Highlights
Burned human skin, which is routinely excised and discarded, contains viable mesenchymal stromal/ stem cells
No significant differences were found in population doubling time, colony formation, cell proliferation cell cycle, production of reactive oxygen species, glycolytic and mitochondrial function, and in the expression of major histocompatibility complex I and II and toll-like receptor (TLR)
Importance, translation: This study reveals valuable insights about Mesenchymal stem cells (MSC) obtained from burned skin and show comparable cellular characteristics with umbilical cord (UC)-MSCs, highlighting their potentials in cell therapy and skin regeneration
Summary
Burned human skin, which is routinely excised and discarded, contains viable mesenchymal stromal/ stem cells (burn-derived mesenchymal stromal/stem cells; BD-MSCs). Dolp et al Stem Cell Research & Therapy (2021) 12:137 possess an ability of self-renewal and cell differentiation, and several other unique characteristics such as secretion of paracrine factors that promote angiogenesis, reepithelialization, granulation tissue formation, and modulate inflammation [3]. They were found to regenerate the epidermis [4] and dermis significantly [5], have anti-inflammatory and anti-fibrotic/scarring [6] potential, and are able to resemble skin pigmentation [7] and renew skin appendages [8]. UC-MSCs are associated with superior benefits compared to other adult MSC sources such as their immunosuppressive properties [20], their multipotency [21], and their ability to accelerate scarless healing [22, 23]
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