Abstract

This article adopts the strategy of adding biochar and increasing HRT to accelerate the performance and particle morphology recovery of anaerobic ammonia oxidation granular sludge stored at room temperature for 68 days. The results showed that biochar accelerated the death of heterotrophic bacteria, shortened the cell lysis and lag period of the recovery process by 4 days, and it only took 28 days for the nitrogen removal performance of the reactor to recover to the original level, and 56 days for re-granulation. Biochar promoted the secretion of EPS (56.96 mg gVSS−1), and the sludge volume and nitrogen removal performance of the bioreactor remain stable. Biochar also accelerated the growth of Anammox bacteria. The abundance of Anammox bacteria in the biochar reactor reached 38.76% on the 28th day. The high abundance of functional bacteria and the optimized community structure of biochar made system (Candidatus_Kuenenia: 38.30%) more risk-resistant than control reactor.

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