Abstract
IN CONNECTION WITH investigations of the endocrine physiology of domestic fowl it appeared essential to determine quantitatively the gonad-stimulating potency of theanterior pituitary. Routine classification of all glands with respect to breed, sex, age,and reproductive condition of the donors frequently resulted in a limited quantity of anterior pituitary substance. The immediate purpose of the present investigation was, therefore, to elucidate a method suitable for the assay of small quantities of material. Pituitaries from males have been used exclusively since larger numbers were available and they have been found to have a higher gonad-Stimulating activity than pituitaries from females (1). The results permit an analysis of the male chicken pituitary type and a determination of the gonadotropic potency of male glands from several different breeds of birds. The determinations also provide a base of reference for comparison of potencies of glands from the female and from birds subjected to various expe...
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