Abstract
A Potyvirus has been isolated from Wedelia trilobata plants in Aligarh district of state Uttar Pradesh (India). The infected plants were showing mosaic and mottling of leaves as shown below. On host range study virus seem to infect many species of Compositae, Umblifereae, Asteraceae, Solanaceae and Euphorbiaceae. The Thermal Inactivation Point (TIP) was between 55-60°C, dilution end point was within 10-4 and Longevity in Vitro (LIV) at 20°C was within 24 hr. The virus was transmitted by Myzus persicae (Sulzer). Cylindrical inclusion bodies were observed in tissue of the infected plants by electron microscopy. The mean length of the flexous particle was 736.5 nm. The virus was successfully purified from infected Wedelia trilobata by differential centrifugation. The purified virus showed the typical UV spectrum of a nucleoprotein with maximum and minimum absorption at 260 nm and 246 nm, respectively. The A260 /A280 ratio was 1.21 ± 0.04. The symptomatic leaves were analyzed through PCR assays with specific primers.
Highlights
Wedelia trilobata is a flowering plant of sunflower family, Asteraceae
In a survey conducted in MarchApril year 2010, Wedelia trilobata plants were found to be heavily infected with virus disease (Figure 1A)
This paper describes a potyvirus isolated from Wedelia in Aligarh district of Uttar Pradesh, in Aligarh district of state Uttar Pradesh (India) and reports the morpho-biological characteristics, purification, physical properties and RT-PCR based detection
Summary
Wedelia trilobata is a flowering plant of sunflower family, Asteraceae. A tropical perennial medicinal herb, with deeply lobed fleshy leaves, growing up to 10 inch tall, spreading like a mat; it makes a dense cover, blossoms profusely, the flowers are orange- yellow. Wedelia trilobata is a medicinal plant used to treat hepatitis infections, to clear the placenta after birth and is used for menstrual pain and unspecified female complaints [1]. In a survey conducted in MarchApril year 2010, Wedelia trilobata plants were found to be heavily infected with virus disease (Figure 1A). Disease symptoms comprised of mosaic and mottling in beginning followed by distortion of leaves in later stages (Figure 1B). Attempts were made to identify the virus causing mosaic and mottling in the experimental plant. This paper describes a potyvirus isolated from Wedelia in Aligarh district of Uttar Pradesh, India and reports the morpho-biological characteristics, purification, physical properties and RT-PCR based detection
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