Abstract
Apple stem grooving virus (ASGV, genus Capillovirus) is disseminated worldwide, usually causing a latent infection in most commercial apple cultivars. However, infected scions grafted onto sensitive material display reduction of yield, loss of fruit quality and tree decline. In Brazil ASGV is associated with severe phloem necrosis, xylem pitting and decline of apple trees on Maruba-kaido (Malus prunifolia cv. Ringo) rootstocks usually in a complex with other latent viruses. Two Brazilian ASGV isolates from a mixed infection causing differing reactions on apple cv. Fuji on Maruba-kaido rootstocks, i.e., normal growth vs. tree decline, have been completely sequenced. The differing intensity of symptoms they co-induced on several woody indicator species/cultivars are indicative of biological differences between the two inocula. Their nucleotide sequences are 92.2% identical and display between 79.2% and 97.1% identity with other ASGV isolates available in GenBank. The two isolates (M219–3 and M220) shared 92.3% deduced amino acid (daa) sequence identity for ORF1, 95.3% for ORF2 and 93.3% for the CP gene. A high degree of daa identity, 95.8% and 96.6%, was observed between the CPs of M219–3 and M220, respectively, with the Brazilian isolate UV01. Potential recombination events and phylogenetic relationships to major parents were investigated in isolates M219–3 and M220.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
