BIOLOGICAL ACTIVITY OF RH IZOSPHERE SOIL SPRING WHEAT IN ASSOCIATION WITH BACTERIA AZOTOBACTER CHROOCOCCUMKZT79. MODIFIED N-ACETYL-D-GLUCOSAMINE

Abstract

Aim. The study of the biological activity of rhizosphere soil of the plants of spring wheat in association with bacteria Azotobacter chroococcum T79, modified N-acetyl-D-glucosamine. Methods. The biological activity of the rhizosphere soil such as the growth-activating ability (method of phytotest), number (by quantitative dilutions of the soil suspension, plating on selective medium growth Eshbi, and counting bacterial colonies) and nitrogenase activity (acetylene method) of nitrogen-fixing microorganisms in the functioning of the association of spring wheat with bacteria Azotobacter chroococcum T79 in combination with N-acetyl-D-glucosamine (0.1 M) in the greenhouse experiments were study. Results. It was shown the significant increase of the number (in 1.4–3.9 times) and nitrogenase activity (in 2.1–5.6 times) of nitrogen-fixing microorganisms during the vegetation of wheat plants as well as the growth-activating ability of the soil (in 1.2 times) in the early stage of formation phytobacteriology association. Conclusion. The treatment of wheat seeds by Azotobacter chroococcum T79, modified N-acetyl-D-glucosamine vs. azotobacter monoinoculant was promoted activation of the functioning of the bacterial nitrogenase (by 10–38 %) and increasing populations of nitrogen-fixing microorganisms (in 1.2–2.2 times) more than their ability to accumulate biologically active substances in the rhizosphere soil of plants.