Abstract
The local study was targeting to investigate the biological antibacterial activity for gradiate concentrations of a local honey bees (125,250, 375 and 500 mg/ml) were tested for five type of bacteria, two of which are gram positive (staphylococcusaureus , streptococcus spp.) and three of them were negative bacteria ( Esherichia coli , salmonella spp. , pseudomonas aeruginosa) by using agar well diffusion method and tube dilution method.The results of agar well diffusion method showed that the bacteria of Staphylococcus aureus and Streptococcus Spp. were the most sensitive to honey dilutions, while E.coli ,Salmonella Spp. showed moderate sensitivity . Pseudomonas aeruginosadid not show any sensitivity. The result also showed that the temperature of incubation temperature of culture media (25and 37 C) had a marked effect on the result of local study, outperformed of diameters of growth inhibition in the cultures that were incubated at 25 C in most of the result recorded.The result of the tube dilution method, Minimum Inhibition Concentration (MIC) was ( 8 , 8 , 125 and 64) mg/ml for the growth of S.aureus , Streptococcus , E.coli and salmonella respectively , while the Minimum Bactericidal Concentration (MBC) for bacteria mentioned were (32 , 64 , 250 and 250) mg/ml respectively for tubes that incubated at 25 C while tubes which incubated at 37 C MIC recorded (8 , 8 , 125 and 250) mg/ml while MBC were ( 32 , 64 , 125 and 250) mg/ml respectively.
Highlights
The results of agar well diffusion method showed that the bacteria of Staphylococcus aureus and Streptococcus Spp. were the most sensitive to honey dilutions, while E.coli,Salmonella Spp. showed moderate sensitivity
ٌطج١عتخ اٌزرو١تت اٌى١ّ١تبةٌٍ ٟغتداس اٌقٍت ٞٛيٚسا ـت ٟؽطبضت١خ اٌغرصِٛتخ ٌزتأص١ر اٌعطتً اٌزضج١طت2 ٟد .ـت١ٔ ٟغ١ر٠تب لعر٠ذ يساضخ ِٓ لجً اٌجبؽش 11 Mogessieد ا ضغٍذ ـٙ١ب ـعبٌ١خ اٌعطً إٌ١غر ٞظد ّٔ ٛطدي ِٓ اٌّّرظتبد اٌّعسٌٚخ ِٓ اٌؽها ِضًٚS.typhimurium , S.enteritidis , E.coliلد رُ رضج١ػ ّٔ٘ٛتب طٕتد اٌزرو١تس %20-15 ـ ٟؽ ٓ١وبْ اٌزرو١س % 10وبـ١ب ٌزضج١ػ ّٔ ٛعرصِٛخ ِّٚ S.aureusتب رغتدس الإقتبسح اٌ١تٗ ذٌت ٝذْ عرصِٛتخ اٌساةفتخ اٌسٔغبس٠خ لد لهٙرد ِمبِٚخ ِررفعخ ارغبٖ رراو١س اٌعطً اٌّؾٍ ٟوبـخ ٘ٚهٖ إٌز١غخ طسزد إٌزبةظ اٌزت ٟرتُ اٌزٛصتً ذٌٙ١ب ِٓ لجً 12 Efemد ٚاٌه ٞضغً ِمبِٚخ عرصِٛخ اٌساةفخ اٌسٔغبس٠خ ٌٍعطتً وّتب ٚعتد اٌجبؽضتبْEl-ٚYagoub 10 Toumد طدَ رؾطص عرصِٛخ اٌساةفخ اٌسٔغبس٠تخ ارغتبٖ ٔتٛطِ ٓ١تٓ اٌعطتً ٘تSunflower ٚ Sun honey ٟ honeyثّٕ١تب اهٙتر ؽطبضت١خ ِعزدٌتخ ارغتبٖ إٌتٛم ٚ Sidir honeyلطتسا اٌجبؽتش ٌته ذٌت ٝذْ اٌقعتبة اٌقتبسعٟ exopigmentationإٌّزظ ِٓ لجً ٘هٖ اٌغرصِٛخ وتبْ ٌتٗ يٚسا ً ِعٕ٠ٛتب ً ـت ٟثمتب ِٚمبِٚتخ عرصِٛتخ اٌساةفتخ ذٌتٝ لعٛا ث١ئ١خ ِعٕ١خ .وّب ٚرعسز ٔزبةظ يساضزٕب اٌؾبٌ١خ ٚثصٛسح طبِخ ِب رٛصٍذ اٌ١خ اٌدساضتبد اٌعٍّ١تخ اٌعبٌّ١تخ اٌزتٟ لعر٠ذ طٍ ٝلٔٛام ِقزٍفتخ ِتٓ اٌعطتً ٚاٌته ٞرتُ عّعتٗ ِتٓ يٚي ِقزٍفتخ 15 ٚ 14 , 13د لهٙترد ٔزتبةظ اٌدساضتخ رجبٚ ٓ٠اظؼ ٌعبًِ اٌزرو١س اٌّطزقدَ ٌّبيح اٌعطً اٌّؾٍ ٟـ ٟاٌزأص١ر طٍ ٝعّ١تع عتراص ُ١الخزجتبس ذ لهٙترد إٌزتبةظ رجبٕ٠ب ً ٚاظؾب ً ٍِّٛٚضب ً ث ٓ١اٌزراو١س اٌّطزعٍّٗ ـت ٟرأص١ر٘تب ـتّٔ ٟت ٛاٌغتراص ُ١ذ ـت ٟاٌٛلتذ اٌتهٌ ٞتُ رعطتِ ٟعبٍِتخ اٌطت١طرح اٌّتب اٌّمطتر د ل ٞرتأص١ر ٠تهور ـت ٟرضجت١ػ إٌّتٔ ٛغتد لْ ز٠تبيح رراو١تس ِتبيح اٌعطتً ساـمتٗ ز٠تبيح ِعٕ٠ٛتخ ذؽصتبة١خ ٚطٕتد ِطتز ٜٛاؽزّبٌ١تخ )
Summary
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