Biological activity of humic substances in the liver of Mongolian gerbils (Meriones unguiculatus)

Abstract

One important reason for non-use of medicinal drugs is their hepatotoxicity. The search for new natural biologically active substances with hepatoprotective properties and which are cost-effective is an important task today. This article presents the data on the impact of the fodder-dietary supplement Humilid alone and in combination with ascorbic acid, and Eco-Impulse on the liver of Mongolian gerbils (Meriones unguiculatus Milne-Edwards, 1867). Hepatomodulation of properties of humic substances in the Humilid confirmed that stimulation of the activity of the enzyme transaminase increases the total amount of protein, especially in combination with ascorbic acid. There was inhibition of accumulation of oxidized products in the cell due to activation of catalase and superoxidedismutase. Intensification of catalase is regarded as one of the important mechanisms of adaptation of the cell under the influence of xenobiotics. Addition of ascorbic acid to the solution Humilid increased the antioxidant action of its active ingredients both through its own antioxidant properties and protection of humic acids from oxidation. Formation of response in liver cells of gerbils receiving active ingredients of the drug Eco-Impulse was accompanied by an increase in the number of TBA-active products and cytochrome C, which indicates the development of oxidative stress, which is accompanied by increased permeability of mitochondrial membranes. The results indicate the hepatoprotective properties of the active ingredients of the drug Humilid, alone and in combination with ascorbic acid, and the need for correcting the concentration of the drug Eco-Impulse for use in clinical practice.