Abstract
The aim of our study was to evaluate GH levels using RIA and bioassay in the 1 months of life. Serum samples from 22 normal full-term newborns were collected at the 5th day and then at the 1st and 4th titis B immunization). In the same serum sample we evaluated both radioimmunological activity of GH and biological activity of GH using Nb2 rat lymphoma cell bioassay. High GH concentratins measured by RIA (32.72±3.95 ng/ml) and Nb2 bioassay (3.29±3.95 U/ml) were observed in neonates at the 5th day of life. At the 1st and 4th month we found a significant decrease (p<0.001) of radioimmunological (of 75, 27% and 92.29%, respectively). A significant correlation (p<0.01) between GH values measured by RIA and Nb2 bioassay was observed at the 5th day, but not at the 1st and 4th month of life, since the biological activity of GH decreased less than the radioimmunological 1 during the 1 months of life. Our results indicate a good agreement between immunoactivity and bioactivity of GH in the first days of life, but not later.
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