Abstract

The activity of trans-2-(2- p-chlorophenyl- trans-ethenyl) cyclopropane carboxylic acid (CPA) as a mimic of abscisic acid (ABA) is reported. CPA had ca. the same inhibitory activity as ABA at 10 −5 M and 10 −6 M in an assay for gibberellic acid-induced α-amylase activity using embryoless barley seeds. At 10 μM, cress seed germination was inhibited by 62%; at 100 μM, lettuce seed germination was inhibited by 58%. ABA completely inhibited seed germination of both species at the respective concentrations. CPA was 1/5 to 1/3 as effective as ABA at equivalent concentrations in inhibiting water loss from detached leaves or intact barley seedlings. However, unlike ABA, at concentrations of 10 −3 M and 10 −4 M CPA inhibited ethylene biosynthesis by soybean hypocotyls. It was concluded that CPA mimics some of the biological functions of ABA at concentrations that are in general higher than ABA. When characterizing the activity of a xenobiotic suspected as being an analogue of a natural plant hormone it is important to complement assays designed to demonstrate activity of the hormone of interest with assays in which activity is unexpected.

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