Abstract
We compared the liver enzyme induction in female rats after subcutaneous injection of 2,3,7,8-tetrachlorodibenzo- p-dioxin (TCDD) and octachlorodibenzo- p-dioxin (OCDD) and correlated the effects with tissue concentrations of the substances. In the first experimental series rats were treated with 150 μg OCDD/kg body wt. Seven days after treatment OCDD concentrations in the liver ranged between 20 and 52 ng/g. No induction of EROD (ethoxy-resorufin-O-deethylase) activity was detectable. In the second series we treated rats daily for 6 consecutive days with 350 μg OCDD/kg body wt. One day after the last application the determination of EROD activity in the hepatic tissue revealed no enzyme induction as compared to vehicle-treated controls. OCDD liver concentrations ranged between 400 and 900 ng/g. Comparatively low concentrations (7 to 28 ng/g) were measured in adipose tissue. In two further experimental series one single dose of 100 ng 14C-TCDD/kg body wt was applied before or during the 6 day OCDD treatment. These results were compared with data from rats treated with TCDD only. TCDD treated rats showed a clear induction of monooxygenases, but no synergistic effect was detectable after the combined treatment. The TCDD liver concentration determined by scintillation counting was 0.75 ± 0.28 ng/g (mean ± S.D.; n = 24). OCDD caused no detectable changes of the TCDD concentration or of the biological effects induced by TCDD.
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