Biological activity and safety profile of monoterpenes against Plutella xylostella L. (Lepidoptera: Plutellidae).

Abstract

Plutella xylostella L. is a cosmopolitan pest of wild and cultivated crucifer vegetables worldwide. It has developed resistance to almost all commercial chemicals, making them one of the most problematic field pests in China. The natural plant extracts and essential oils (EOs) could be a safe alternative for agricultural pests. The development and production of EOs decrease the negative effects of synthetic chemicals. In the present study, the fumigation activity of 8 pure monoterpenes against P. xylostella was evaluated. Results from fumigation tests revealed that 8 tested compounds exhibited various degrees of toxicity against adults of the diamondback moth. Cuminaldehyde was the most toxic compound based on the 12-h LC50 (0.17mg/L) and 24-h LC50 (0.12mg/L) values, respectively. Also for larvae and eggs, cuminaldehyde was the most toxic compound. The 12-h LC50 value for cuminaldehyde to 1st, 2nd, and 3rd instar larvae was 0.10mg/L, 0.12mg/L, and 0.55mg/L, respectively. The 24-h LC50 value for the different instar larvae was 0.07mg/L, 0.09mg/L, and 0.35mg/L, respectively. The 24-h LC50 value for eggs (endpoint hatching rate) was 1.95mg/L for cuminaldehyde, followed by carvacrol and eugenol (2.05mg/L and 2.31mg/L, respectively). Cuminaldehyde was very friendly to the larvae and adults of Harmonia axyridis and did not cause any mortality. Our results indicated that cuminaldehyde had potential insecticidal activity against P. xylostella and could be utilized in the novel biological pesticide development.