Biological Activity and Optical Sensor Properties of Green Synthesis Polymer

Abstract

AbstractIn this study, 4‐amino‐3‐hydroxy‐1‐naphthalene sulfonic acid (Monomer) was converted to its enzyme‐catalyzed oxidative polymerization (Poly‐enz) in dioxane solvent by horseradish peroxidase enzyme (HRP) in the presence of hydrogen peroxide as oxidant. For the analysis of the monomer and its synthesized Poly‐enz, 1H‐NMR, 13C‐NMR, FT‐IR, TGA, and SEM measurements were determined. In addition, the optical ion sensor properties of Poly‐enz against metals such as Ag+, Cd2+, Co2+, Hg2+, Ni2+, Pb2+, Zn2+, Cu2+ were examined with UV‐Vis spectrophotometer. It was observed that Cu metal showed sensory properties. DNA cleavage and DNA binding activities of monomer and Poly‐enz were done using by agarose gel electrophoresis and UV‐Vis methods, respectively. It was seen that the Poly‐enz interacted with DNA electrostatically. This enzymatic polymer was found to cleave pBR322 plasmid DNA both hydrolytically and oxidatively as well. The antioxidant activities of the compounds were examined by three different methods (DPPH, ABTS, FRAP) and it was found that the Poly‐enz showed high antioxidant activity compared to its monomer. Antimicrobial activities were studied against various bacterial and yeast cultures by the minimum inhibition concentration (MIC) method. It was observed that the antimicrobial activities of the enzymatic polymer in different microorganisms increased depending on the concentration.